Figure 5From: Necdin modulates proliferative cell survival of human cells in response to radiation-induced genotoxic stress The effect of Necdin depletion on premature senescence. (A) Protein expression level in IMR90 cells showed efficient depletion in Necdin expression by shRNA and overexpression of RasV12, which served as a positive control of senescence induction. (B) Senescence-associated β-galactosidase staining 14 days after lentiviral infection demonstrated senescence only in RasV12-expressing cells. (C) Quantification of senescent cells represented by the average of SA-β-gal positive cells. Mean of six different fields ± standard deviation.Back to article page