Figure 3

Analysis of apoptosis induction in RH30 and RD Rhabdomyosarcoma cells. To assess the role of autophagy in drug-treated RH30 and RD cells, apoptosis was measured by flow- cytometry after staining treated and untreated cells (Bortezomib 7,5nM; 17-DMAG 50nM; Bortezomib/17-DMAG) with Annexin-V-FITC/Propidium Iodide (AV/PI) in the presence or absence of non-toxic concentrations of rapamycin (5 ng/mL). Non-apoptotic (healthy, AV⁻/PI⁻), apoptotic (AV⁺/PI⁻ and AV⁺/PI⁺) and necrotic cells (AV⁻/PI⁺) were identified, and their relative percentages reported in the corresponding quadrants. The relative amount of each fraction is graphed aside.