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Figure 6 | BMC Cancer

Figure 6

From: The heat shock protein-90 co-chaperone, Cyclophilin 40, promotes ALK-positive, anaplastic large cell lymphoma viability and its expression is regulated by the NPM-ALK oncoprotein

Figure 6

Cyp40 does not regulate NPM-ALK expression or appear to influence NPM-ALK signalling. (A) Western blot analysis of NPM-ALK protein levels (anti-ALK blots) in lysates collected from Karpas 299 and SUP-M2 cells after transfection with pooled control or Cyp40 siRNA. (B) The tyrosine phosphorylation status of NPM-ALK was analyzed by immunoprecipitation of NPM-ALK (anti-ALK IP) followed by anti-phosphotyrosine (pTyr) western blotting in cells treated as in (A). (C) The tyrosine phosphorylation of total cellular proteins (anti-pTyr blot), the phosphorylation status of STAT3 (anti-pSTAT3 blot), and total Akt levels were analyzed by western blotting in lysates from cells transfected with pooled control, Cyp40, or ALK siRNA.

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BMC Cancer

ISSN: 1471-2407

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