Figure 3

Bisulfite sequencing of the KDR promoter. A CpG island is located between -1231 and 1125 relative to the ATG codon of KDR. The 3' part of the KDR promoter region and exon 1 (612 bp, 53 CpG sites) were sequenced after bisulfite conversion of DNA from cell lines DOHH-2 (KDR negative) and HEL (KDR positive) as well as from HDMECs and HUVECs. Each line depicts a sequenced clone representing the methylation status of an individual allele. CpGs are represented as open dots (if unmethylated) or filled dots (if methylated). Results of qRT-PCR and methylation specific PCR (MSP) are shown on the left hand side. M: signal in M-PCR; U: signal in U-PCR.