Figure 7From: DcR3 binds to ovarian cancer via heparan sulfate proteoglycans and modulates tumor cells response to platinum with corresponding alteration in the expression of BRCA1 DcR3 modulates response to platinum in ovarian cancer cell lines. A and B, Chronic exposure to DcR3 in culture (12 weeks) resulted in increased resistance to cis-platinum in SKOV-3 and OVCAR-3 cells with a mean of 15% more viable cells across a wide range of very high platinum doses, p < 0.001 by ANOVA. C. Photomicrographs demonstrate that DcR3 exposed SKOV-3 cells remain adherent and alive 48 h after platinum has been removed from the cultures where cell numbers continue to decline after platinum has been removed from the parent SKOV-3 cell line. DcR3 treated cells were 60% viable at 48 h by eosin stain. D. CaOV-3 cells exposed to DcR3 under identical conditions demonstrated increased cytotoxicity in response to platinum with > 25% more cell death even at the lower doses, p < 0.001 by ANOVA. Insets indicate that DcR3 exposure has no significant effects on the growth rate of the cell lines tested.Back to article page