Figure 6
DcR3 binding to EOC is heparin sulfate dependent. A. DcR3 binding is completely inhibited by heparin sulfate in all three cell lines. B. DcR3 binding is also inhibited by the removal of heparan sulfates from HSPGs on the cells surface with heparinase or by removal of complete HSPGs with their peptide backbone by trypsin. C. The surface adhesion molecule EpCAM was used as a control for heparinase and trypsin treatments with appropriate responses seen.