Figure 2

Generation of intracellular ROS in ovarian cancer cells after individual or combinational treatment with TM and doxorubicin. (a) SKOV-3 cells were treated with TM (0, 30 μM) for 24 h, after which the cells were treated with doxorubicin (0, 10 μM) for another 5.5 h. Generation of intracellular ROS was measured by flow cytometry (see Methods). Data are presented as relative fluorescence intensity (DCF). (b) SKOV-3 cells were treated with TM (0, 30 μM) for 24 h, after which the cells were treated with doxorubicin (0, 5 μM) for another 24 h in the presence or absence of radical scavenger ascorbic acid (750 μM) before the viability of cells was measured. Data are expressed as the mean of the triplicate determinations (X ± SD) compared to untreated cells [100%].