Figure 4

Overexpression of HOXA1 induces cellular proliferation. (A) Representative duplex RT-PCR and western blot analysis of HOXA1 in HaCAT-Control and HaCAT-HOXA1 transfectants, revealing an increase in HOXA1 levels in overexpressing clones. Assays measuring BrdU incorporation (B) and Ki67 expression (C) demonstrated that HOXA1 overexpressing cell lines have a statistically significant increase in proliferation as compared to control cells (for BrdU index p < 0.01 between groups, and for Ki67 index p < 0.05 between groups). The labeling index of BrdU and Ki67 corresponds to the mean percentage of positive cells of 3 experiments for each cell line.