Table 2 Primers for quantitative real-time RT-PCR
From: Prognostic impact of mRNA levels of osteopontin splice variants in soft tissue sarcoma patients
Gene | Primer | Sequence 5' → 3' | Localization | Annealing temperature | |
|---|---|---|---|---|---|
HPRT | HPRT309 | 5'-TTGCTGACCTGCTGGATTAC-3' | sense | 391-410 | 58°C |
HPRT507 | 5'-CTTGCGACCTTGACCATCTT-3' | antisense | 633-652 | ||
OPN-a | OPN-a fw 323 | 5'-ATCTCCTAGCCCCACAGAAT-3' | sense | 323-342 | 58°C |
OPN-a rev 508 | 5'-CATCAGACTGGTGAGAATCATC-3' | antisense | 529-508 | ||
OPN-b | OPN-b fw 323 | 5'-ATCTCCTAGCCCCACAGAC-3' | sense | 323-341 | 62°C |
OPN-b rev 509 | 5'-AAAATCAGTGACCAGTTCATCAG-3' | antisense | 531-509 | ||
OPN-c | OPN-c fw 246 | 5'-TGAGGAAAAGCAGAATGCTG-3' | sense | 246-265 | 58°C |
OPN-c rev 377 | 5'-GTCAATGGAGTCCTGGCTGT-3' | antisense | 396-377 |