Figure 2

The inhibitory effects of baicalein on Ezrin and phos-Ezrin in A431 cells. a. A431 cells were treated with baicalein at 0, 10, 20, and 40 μM for 24 h. b. A431 cells were treated with 20 μM baicalein for 12, 24, and 48 h. After baicalein treatment, total protein was extracted from these cell samples. The protein samples were then subjected to western blotting, and the levels of Ezrin and phos-Ezrin expression in these samples were determined. Baicalein inhibited the expression of Ezrin and phos-Ezrin at Thr-567 in a dose- and time-dependent manner. β-actin or Ezrin respectively served as the control for quantifying Ezrin or phos-Ezrin. Three independent experiments were carried out and representative figures are shown.