Figure 6

Validation of gene array findings by real time PCR. Figure 6A: STAT 1 mRNA expression in control cells transduced with non targeting shRNA (NT) [shown in all diagrams in Figure 6 as NET1 (+)] and knockdown cells transduced with NET1 targeting shRNA [shown in all diagrams in Figure 6 as NET1(-)] Figure 6B: TSPAN 1 expression in control cells transduced with non targeting shRNA (NT) cells and NET1 knockdown cells transduced with NET1 targeting shRNA. Figure 6C. TGFBi expression in control cells transduced with non targeting shRNA (NT) and NET1 knockdown cells transduced with NET1 targeting shRNA. p < 0.0001 Figure 6D. CCL5 mRNA expression in control cells transduced with non targeting shRNA (NT) and knockdown cells, p < 0.0001; Figure 6E. EGFR expression in untreated and LPA treated AGS cells. Figure 6F: PPARD expression in untreated and LPA treated AGS cells. Figure 6G: IGF1R expression in LPA treated cells tranduced with non targeting shRNA control cells and LPA treated NET1 knockdown cells transduced with NET1 targeting shRNA, p = 0.0001. Figure 6H. PIP5K3 expression in LPA treated cells tranduced with non targeting shRNA control cells and LPA treated NET1 knockdown cells transduced with NET1 targeting shRNA. P < 0.0001.