Figure 4From: Characterization of a novel PTEN mutation in MDA-MB-453 breast carcinoma cell line Ubiquitination of PTEN E307K by NEDD4-1. Recombinant WT and E307K PTEN were incubated with either Ub-WT (A) or Ub-KO (B) under the conditions indicated. Reactions were resolved on SDS-PAGE and probed with an anti-PTEN polyclonal antibody (#9552). The amount of PTEN in each reaction is shown (lower panels).Back to article page