Figure 3

siRNA mediated downregulation of PDGFRs. RJ348 and RJ423 recurrent cell lines were plated and 48 h later they were transiently transfected with oligonucleotides specific for either PDGFRα, β or both; an appropriate GC oligonucleotide was used as a control. Seventy-two hours post-transfection, protein was collected and subjected to western blotting probing for both PDGF receptors as well as pAkt, Akt, pErk1/2, Erk1/2 and cyclin D1. β-actin served as a loading control.