Figure 1

GnRH receptor is expressed across a wide range in breast cancer and is highest in triple negative tumours when measured by immunostaining. Association of GnRH receptor expression and (A) Cancer type and (B) Cancer grade. Quantitative immunofluorescence (AQUA) was used to measure GnRH receptor. One way ANOVA was used to test for significant differences between subtypes, the mean for each group is shown with a dashed line. C. Representative examples of high GnRH receptor expression (top images) and low expression (bottom images). Left hand images are immunohistochemical images of tissue microarray (TMA) cores of individual breast cancer with brown staining corresponding to GnRH receptor expression and blue to haematoxylin staining. Right hand images are immunofluorescence images of TMA cores, with red staining corresponding to GnRH receptor expression, blue (DAPI) staining indicating cell nuclei and green staining detecting cytokeration (ie carcinoma cell) staining. White arrows indicate areas of positive expression.