Figure 1

Murine and human tumor cells are susceptible to α-TEA irrespective of HER2/ neu status. (A) HER2/neu expression was determined by flow cytometry. The murine MMC and ANV tumor cells were stained with a rat-HER2/neu-specific antibody (7.16.4) and then with a FITC-conjugated anti-mouse-IgG antibody. The human MDA-MB-453 and MDA-MB-231 tumor cells were stained using a FITC-conjugated human-HER2/neu-specific antibody. Cells were gated on light scatter. (B) Tumoricidal effect of Vα-TEA. Tumor cells were allowed to adhere overnight and were then treated with media alone or increasing doses of Vα-TEA. Cell survival was determined by MTT assay after 24 h (MMC, ANV), 48 h (MDA-MB-231) or 72 h (MDA-MB-453). Results are expressed as mean percentage ± SD of untreated cells (media alone). Non-linear regression analysis was performed to determine half-maximal inhibitory concentrations (IC₅₀).