Figure 7
Role of calcium in phosphorylation of ERK, Akt, and Shc. A, Effect of thapsigargin (Thap) and neurotensin (NT) on phosphorylation of ERK, Akt, and Shc in HCT116 cells. Quiescent cells were pretreated with vehicle (0.9% NaCl) or cetuximab (25 μg/ml) for 30 min before stimulation with the vehicle (0.5% DMSO), 1 μM NT, or 1 μM Thap for five min. Cells were harvested for subsequent Western analysis as described in Methods. Results represent one typical of three independent experiments. B, Effect of PI3Kβ inhibitor on NT-stimulated ERK and Akt phosphorylation in Panc-1 cells. Quiescent cells were pretreated with vehicle (0.5% DMSO) or 1 μM TGX-221 for 30 min before stimulation with 10 nM EGF or 1 μM neurotensin (NT) for 5 min. Cells were harvested for subsequent Western analysis as described in Methods. Results represent one typical of four experiments.