Table 1 HRM primer sequences
Gene | Exon | Primer sequence | Amplicon size |
|---|---|---|---|
KRAS | 2 | F: 5'-GGCCTGCTGAAAATGACTGAATATAA-3' | 170 bp [26] |
R: 5'-AAAGAATGGTCCTGCACCAGTA-3' | |||
BRAF | 15 | F: 5'-TCATGAAGACCTCACAGTAAAAATAGG-3' | 164 bp |
R: 5'-AGCAGCATCTCAGGGCCAAA-3' | |||
EGFR | 19 | F: 5'-GTGCATCGCTGGTAACATCCA-3' | 250 bp§ [27] |
R: 5'-AAAGGTGGGCCTGAGGTTCA-3' | |||
EGFR | 20 | F: 5'-ACCTCCACCGTGCA(T*)CTCAT-3' | 128 bp |
R: 5'- ATTACCTTTGCGATCTGAACACACC -3' | |||
EGFR | 21 | F: 5'-CCTCACAGCAGGGTCTTCTCTG-3' | 210 bp§ [27] |
R: 5'-TGGCTGACCTAAAGCCACCTC-3' |