Figure 4

Equivalent DISC protein recruitment, but not caspase 8 activation, following agonistic DR4 and DR5 antibody treatment. A: Analysis of the DR4 and DR5-DISC in SW948. Cells were incubated for 15 min with agonistic DR4 and DR5 antibodies before co-immunoprecipitation of the associated DISCs using protein G-agarose beads, and subsequent analysis by Western blotting using antibodies directed against DR4, DR5, FADD, caspase 8 and c-FLIP. Post = antibodies added after cell lysis; Stim = cells stimulated with the indicated antibody for 15 min. One representative of at least two independent experiments is shown. B: Time-dependent cleavage of pro-caspase 8 in SW948 cells treated with 50 nM agonistic DR4 and DR5 antibodies.