Figure 4
Adhesion of prostate cancer cells to extracellular matrix proteins. PC-3, DU-145 or LNCaP cells were treated with 1 μM AEE788, 1 mM VPA or 1 nM RAD001, or with the drug combination (triple). Non-treated cells served as the controls. Cells were then added to immobilized collagen, laminin, or fibronectin at a density of 0.5 × 106 cells/well for 60 min. Plastic dishes were used to evaluate unspecific binding (background control). Non-adherent tumor cells were washed off in each sample, the remaining cells were fixed and counted in five different fields (5 × 0.25 mm2) using a phase contrast microscope. Mean values were calculated from the five counts. Specific adhesion capacity (background adhesion on plastic surface was subtracted from adhesion to matrix proteins) is depicted as bound cells/mm2. One representative of six experiments is shown. *indicates significant difference to controls, #indicates significant difference to single drug treatment.