Figure 4
VEGF 121 /rGel mediates inhibition of angiogenesis in chicken embryo CAMs via reduction of the vascular area and number of vascular branches. A, Vasculature of a CAM after stimulation with bFGF alone. B, rGel had no effect on angiogenic stimulation of bFGF at either 1 nM or 10 nM. C, 1 nM VEGF121/rGel in the presence of 50 ng of bFGF inhibited angiogenesis. D and E, Quantitative evaluation of VEGF121/rGel-mediated inhibition of angiogenesis in the CAM model, normalized to CAMs treated with buffer (PBS; equal to 100%). D, VEGF121/rGel at both 1 and 10 nM decreased the vascular area. As expected, rGel alone had no effect. E, VEGF121/rGel decreased the number of newly sprouting vessels. VEGF121/rGel at a concentration of 1 nM dramatically affected the formation of the neovasculature, completely inhibiting bFGF-mediated stimulation of the neovasculature. As expected, rGel did not affect the number of newly sprouting vessels. Data shown represent the means ± standard deviations from replicated experiments. *, P < 0.001; t-test, double-sided.