Figure 7From: Downregulation of TFPI in breast cancer cells induces tyrosine phosphorylation signaling and increases metastatic growth by stimulating cell motility Phosphorylation status of signaling molecules in the total lysate of transduced cells after FBS stimulation. Cells were starved for 5.5 hours before 1-2 × 106 cells were stimulated with 10% FBS for indicated time points. General tyrosine phosphorylation in Sum102 (A) and MDA-MB-231 (B) cells expressing shRNA6 (lane 1), -7 (lane 2) or empty vector control (lane 3) and (C) in MDA-MB-231 cells expressing shRNA7β (lane 1), -9β (lane 2) or empty vector control (lane 3), detected using anti-4G10 antibody. Src family phosphorylation in MDA-MB-231 cells expressing (D) shRNA6 (lane 1), -7 (lane 2) or empty vector control (lane 3) and (E) shRNA7β (lane 1), -9β (lane 2) or empty vector control (lane 3), detected using anti-phospho-Src antibody. c-Src was used as loading control. One representative experiment of three is shown.Back to article page