Figure 5From: Downregulation of TFPI in breast cancer cells induces tyrosine phosphorylation signaling and increases metastatic growth by stimulating cell motility Invasion of transduced cells measured using modified Boyden chambers. Cells were seeded in matrigel pre-coated chambers and incubated for 48/24 hours (Sum102/MDA-MB-231) at 37°C before stained with Calcein AM. Results are presented as mean relative invasion + SEM of three independent experiments. (A) Sum102 cells (n ≥ 8, **p = .0063 and ***p ≤ .0001 against pSiRPG) and (B) MDA-MB-231 cells (n ≥ 8, ***p < .0001 against pSiRPG) with TFPI downregulated. (C) MDA-MB-231 cells with TFPIβ downregulated (n ≥ 8, *p = .0227 and **p = .0098 against pSiRPG).Back to article page