Serum estradiol levels associated with specific gene expression patterns in normal breast tissue and in breast carcinomas

Table 1 Genes significantly differentially expressed in normal breast tissue of healthy women according to serum estradiol.

	Gene Name	SCGB3A1	SLITRK4	TLN2	DCLK1	RSPO1	PTGS1
A	Chromosomal location of the gene	5q35.3	Xq27.3	15q15-q21	13q13	1p34.3	9q32-q33.3
	q-value (%) SAM¹⁾	0	0	0	0	0	0
	Gene expression in high s-est²⁾
	(compared with low s-est)	up	up	up	up	up	down
B	BC³⁾ vs normal breast tissue (p-value)⁴⁾	5.00E-15	1.50E-03	2.60E-04	6.00E-04	4.90E-12	0.02
	Gene expression n BC³⁾
	(compared with normal tissue)	down	down	down	down	down	up
	ER+ BC vs normal tissue^{5) 4)}	2.20E-13	0.01	1.30E-03	4.20E-03	3.30E-12	0.05
	Gene expression in ER+ BC
	(compared with normal tissue)	down	down	down	down	down	up
	ER- BC vs normal tissue^{6) 4)}	1.10E-04	0.03	0.01	0.01	0.02	0.05
	Gene expression in ER- BC
	(compared with normal tissue)	down	down	down	down	down	up
	Invasive BC vs DCIS^{3) 4)}	0.04	0.12	0.01	0.66	0.24	0.001
	Gene expression in invasive BC
	(compared with DCIS)	down	-	up	-	-	up

A) Q-values and regulation of gene expression from quantitative SAM analysis of gene expression according to serum estradiol. B) Significance testing of difference in gene expression of the genes identified in A) in different sample cohorts.
1) Q-value from SAM of gene expression in normal breast tissue according to serum estradiol
2) s-est = serum estradiol
3) BC = breast cancer
4) P-value from two-sided t-test
5) ER+ BC = estrogen recepor positive breast cancer (n = 53)
6) ER- BC = estrogen recepor negative breast cancer (n = 8)

ISSN: 1471-2407