Figure 3

AFMC increases DR5 protein levels in A549 cells. Figure 3A: A549 cells were treated with the indicated concentrations of AFMC for 24 h, and DR5 levels were measured by Western blotting. β-actin was used as a loading control. Figure 3B: A549 cells were treated with 1.0 μmol/L AFMC for the indicated time, and DR5 levels were measured by Western blotting. β-actin was used as a loading control. Figure 3C: A549 cells were treated with the indicated concentrations of AFMC for 24 h, DR5 and DR4 protein levels on the surface of A549 cells were determined using flow cytometry. Mouse IgG1 was used as an isotype control.