Figure 1

Cytotoxic and apoptotic activities of AFMC or TRAIL or both on A549 cells. Figure 1A: A549 cells were treated with AFMC for 30 min and then treated with or without TRAIL for 24 h at the indicated concentrations. Cytotoxicity was assessed by ELISA. Columns, average of three individual experiments; bars, SD. Figure 1B: A549 cells were treated with chrysin for 30 min and then treated with or without TRAIL for 24 h at the indicated concentrations. Cytotoxicity was assessed by ELISA. Columns, average of three individual experiments; bars, SD. Figure 1C: A549 cells treated with 1 μmol/L AFMC or 30 ng/mL TRAIL for 24 h or the pretreated with 1 μmol/L AFMC for 30 min followed by 30 ng/mL TRAIL for 24 h. DNA content of the cells was analyzed by flow cytometry. Columns, average of three individual experiments; bars, SD. a P < 0.05 vs 0.2% DMSO; b P < 0.05 vs 30 ng/mL TRAIL or 1.0 μmol/L AFMC alone. Figure 1D: A549 cells were pretreated with 1.0 μmol/L AFMC for 30 min and then treated with 30 ng/mL TRAIL for the indicated times. DNA content of the cells was analyzed by flow cytometry. Figure 1E: A549 cells were treated as Figure 1C described, fragmented DNA was extracted from treated cells and analyzed on a 2.0% agarose gel.