Figure 9

Inhibition of MMP-2 and MMP-9 secretion and activation MAPK signaling pathway. A) MCF-7/CD44st cells were pretreated (+) or unpretreated (-) with CD44 antibody, PD98059 and manumycin A, and then stimulated with HA (+) or without HA (-) for the indicated concentrations and time periods. Conditioned medium samples were harvested and subjected to zymography (top panel). B) MCF-7/CD44st cells were pretreated (+) or unpretreated (-) with CD44 antibody, PD98059 and manumycin A, for the indicated concentrations and time periods, and then incubated with 100 μg/ml of HA (+) or without HA (+) for 0.5 h. Cells lysates were subjected to immunoblotting with anti-phospho-ERK (bottom panel).