Figure 6From: A high-throughput protocol for mutation scanning of the BRCA1 and BRCA2genes Detection of a sequence variation immediately adjacent to the 3' end of a primer. The class 1 SNP (BRCA2 c.1365A>G) (red) is readily detectable at the position immediately adjacent (1 base pair) to the 3' end of the reverse primer in this 232 bp amplicon (BRCA2 exon 10C).Back to article page