Figure 5

Two variations within the same sample result in greater HRM differences. ( BRCA1 exon 13). The left panel shows the difference plot where 3 samples have been compared to the wildtype controls (grey). The green profile represents the a heterozygote for common polymorphism c.4308T>C while the blue represents the mutation c.4327C>T. The presence of both sequence variants (red) results in greater instability than each individual sequence variation due to the double mismatch in heteroduplexes. The right panel with melting peak curves show the complex melting nature of two co-existing sequence variants (red).