Figure 6
Matrix-mediated Panc-1 invasive phenotypes are regulated by β 1 -integrin. To determine the engagement of integrins for the invasiveness of Panc-1 in FAP+matrix, time-lapse acquisition assays were performed in the presence of functional blocking β1-integrin antibody mAb13 and blocking peptide ATN-161. (A) Panels are shown representative examples of migration tracks of individual cells invading through assorted matrices. (B) Distinct responses of Panc-1 cells to the integrin inhibition were measured by average velocity and directionality. Compared to the control experiment using rabbit serum, inhibition of β1-integrins significantly attenuated both velocity (67% inhibition, p < 0.001) and directionality (70% inhibition, p < 0.001) of Panc-1 cells in FAP+ but not in FAP-matrix (p = 0.07 and p = 0.06, respectively). In addition, specific inhibition of α5β1-integrin decreased both velocity (43% inhibition, p = 0.002) and directionality (53% inhibition, p < 0.001) of Panc-1 cells on FAP+ matrix. Statistical significances were marked using asterisks.