Figure 1

DEK expression in cell lines. A. Whole cell lysates were prepared from bladder cancer cell lines (RT-4, 5637, T-24 and TCCSUP), Urothelial cell line (UroTSA) and undifferentiated and differentiated progenitor bladder epithelial cell lines. DEK protein was evaluated by western blot analysis using a DEK monoclonal antibody (BD Bioscience). β-actin was used as a loading control. B. Expression of DEK protein in whole cell (WC) lysate and respective conditioned media from 5637, 5637 expressing nonspecific shRNA (Ns shRNA), DEK shRNA or overexpressing DEK with a V5 tag (DEK-V5) by western blot assay using a monoclonal DEK antibody. Bottom panel represents a Coomassie stained gel to show that equal amounts of proteins were loaded on the gel.