Figure 4

Treatment with 5-AzaC and TSA rescues SIP1 expression in HCC cell lines. The restoration of SIP1 expression is analyzed by multiplex semi-quantitative RT-PCR. (A) Treatment of HepG2 cells with 5-AzaC (2.5 μM) and TSA. Mahlavu cells are included as a positive control of SIP1 expression. DMSO is used as a control of TSA treatment and a DNA-free PCR mixture [(-)] is also tested. (B) PLC and Hep3B cell lines are treated with 2.5 μM 5-AzaC and 1 μM TSA. The SkHep1 cell line was used as a positive control. [(-)]: DNA-free PCR mixture. M: marker (bp). In both (A) and (B), bands lower than 100 bp are primer dimers.