Figure 5From: Synergistic effects of oncolytic reovirus and docetaxel chemotherapy in prostate cancer Enhanced apoptotic cell death of PC3 cells treated with reovirus and docetaxel in combination. PC3 cells were treated with reovirus MOI 1 (open bars), 20 nM docetaxel (wide diagonal stripes) or both agents together (narrow diagonal stripes) for 24 h (a) or 48 h (b) before staining with Annexin V (A) and propidium iodide (PI) and analysing using flow cytometry. Untreated cells (dark grey bars were included as a negative control). Addition of each agent alone resulted in an increased late apoptotic/necrotic population (A+/PI+) and concomitant reduction of intact cells (A-/PI-) compared to untreated cells. There was a dramatic increase in this effect at 48 h when the two agents were combined.Back to article page