Figure 3From: Synergistic effects of oncolytic reovirus and docetaxel chemotherapy in prostate cancer Live virus is required for efficient cell kill and enhanced cell kill with docetaxel. PC3 cells were treated with live (diamonds), heat-inactivated (squares), or UV-inactivated (triangles) virus for 48 h at the MOI indicated at which point survival was measured by MTS assay (a). PC3 cells were also treated with docetaxel alone (diamonds), or docetaxel with live reovirus (squares), heat-inactivated reovirus (triangles) or UV-inactivated virus (crosses) for 48 h at the concentration and MOI indicated. Survival was measured at this time by MTS assay. The combination of live reovirus with docetaxel enhanced cell kill compared with docetaxel alone (P < 0.0001, two-way ANOVA), however this enhancement of cell kill was not apparent when heat inactivated or UV-inactivated reovirus was used (b). Data for both are normalized to uninfected controls (MOI 0) and are representative of three independent experiments.Back to article page