Figure 2From: Genistein inhibits proliferation of colon cancer cells by attenuating a negative effect of epidermal growth factor on tumor suppressor FOXO3 activity Genistein inhibits FOXO3 phosphorylation via PI3K/Akt. Total protein from HT-29 cells (control and EGF treated for 30 minutes) was examined for phosphorylated (A) EGFR and (B) Akt in the presence and absence of genistein. Genistein inhibits EGF induced Akt phosphorylation in HT-29 cells. The experiment was repeated in triplicate and graphs represent densitometric analysis (n = 3, *p < 0.05).Back to article page