Figure 14

Effect of huMETCAM/MUC18 expression on in vitro invasiveness of MDA-MB-231 and MDA-MB-468 cell lines (24 hours). The invasiveness of MDA-MB-231 and MDA-MB-468 cells was determined 24 hours after seeding the cells as described in "Materials and Methods" by coating with 150 μg of Matrigel and using a pore size of 8 μm. 7.5 to 15 μg/ml of the anti-huMCAM/MUC18 antibody (open columns) or the isotype control antibody (chicken IgY) (filled columns) was added to block the invasiveness of these clones. Means and standard deviations of triplicate values of the test are shown.