Figure 7From: The novel curcumin analog FLLL32 decreases STAT3 DNA binding activity and expression, and induces apoptosis in osteosarcoma cell lines Inhibition of caspase activation did not affect loss of STAT3 following FLLL32 treatment. A) Canine (OSA8) and human (SJSA) OSA cell lines were pretreated with the pan-caspase inhibitor 80 μM Z-VAD-FMK or DMSO for 2 or 24 hours then treated with DMSO or 10 μM FLLL32 for 18 hours. Protein lysates were generated and separated by SDS-PAGE and western blotting for STAT3, PARP, and β-actin was performed. Experiments were repeated two times. B) Canine (OSA8) or human (SJSA) OSA cell lines were pretreated with the pan-caspase inhibitor 80 μM Z-VAD-FMK, DMSO, or media for 2 or 24 hours then treated with media, DMSO, or 10 μM FLLL32 for 18 hours. Caspase-3/7 activity was measured using the SensoLyte® Homogeneous AMC Caspase-3/7 Assay kit. Experiments were performed in triplicate and repeated two times.Back to article page