Figure 5
mRNA expression of genes with regard to cell cycle. A, RNase protection assays were performed using RNA from SGC7901 cells treated with either 10 mM HMBA, 20 mM LiCl, or a combination of HMBA and LiCl for 24 h, hybridized with multi-probes for cell cycle dependent kinase inhibitors (A; hCC-2) or cyclins (B; hCYC-1). B, SGC7901 cells were pre-treated with (+) or without (-) 10 mM LiCl (right) or 10 μM SB-415286 (left) for 30 min followed by combination treatment with 10 mM HMBA for 24 h. Whole cell protein extracts were resolved on SDS-PAGE, transferred to PVDF membranes, and immunoblotted with antibodies to p27Kip1, p21Waf1, CDK2, or β-actin.