Figure 4

Expression of Ape1/Ref-1 in normal, BH, DCIS and IBC tissue. A. Tissues were subjected to IHC using an Ape1/Ref-1-specific antibody. Representative slides are shown at 40× magnification. B. The relative mean intensity was determined from 6-8 images for each BH (n = 13), DCIS (n = 13) or IBC (n = 22) section and is shown graphically. ANOVA was used to detect statistical differences in the relative expression of BH, DCIS (p = 0.0002) and IBC (p < 0.0001) compared to normal mammary tissue, where normal mammary tissue is equal to one (dashed line). C. Cellular localization of Ape1/Ref-1 expression was determined for each tissue section, classified by disease state and shown graphically (N, nuclear; C, cytoplasmic; N+C, nuclear and cytoplasmic).