Figure 2
Effects of various subeffective doses of γ-tocotrienol (γT 3 ) when given alone or in combination with subeffective dose of erlotinib (E), gefitinib (G), or trastuzumab (T) on neoplastic +SA mammary epithelial cell growth. Cells were plated at a density of 5 × 104 cells per well (6 replicates/group) in 24-well plates and exposed to respective control and treatment serum-free defined media for a 4-day period. Viable cell number was determined by MTT colorimetric assay and indicated as percentage of control. Each bar represents the mean ± SEM in each treatment group and is representative of three independent experiments. *P < 0.05 as compared to the vehicle treated controls.