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Table 5 Comparison of qPCR data and NotI-microarrays for RBSP3 gene

From: Simultaneous down-regulation of tumor suppressor genes RBSP3/CTDSPL, NPRL2/G21 and RASSF1A in primary non-small cell lung cancer

Sample number

qPCR

bNot I-microarrays

Possible reason of mRNA decrease

 

mRNA

decrease

aDNA

copy number

  

AC

1

0.39

+

+

deletion

2

0.15

-

+

methylation

4

0.29

-

-

another mechanism

5

0.49

+

+

deletion

7

0.37

-

-

another mechanism

11

0.12

-

-

another mechanism

16

0.29

-

+

methylation

17

0.12

-

+

methylation

SCC

23

0.16

-

+

methylation

25

0.13

+

+

deletion and methylation

26

0.21

-

+

methylation

27

0.39

+

+

deletion and methylation

43

0.48

-

+

deletion

46

0.14

-

+

methylation

52

0.06

-

+

methylation

53

0.46

-

+

methylation

54

0.08

-

+

methylation

55

0.26

-

-

another mechanism

  1. a + deletion, - retention
  2. b+ deletion and/or methylation, - no changes