Simultaneous down-regulation of tumor suppressor genes RBSP3/CTDSPL, NPRL2/G21 and RASSF1A in primary non-small cell lung cancer

Table 3 The frequency of mRNA decrease (FD) and the average level of mRNA decrease (LD_av)

Clinical stage/Groups		Frequency (FD)^aand level of mRNA decrease (LD_av)^b
		RBSP3 (59 tumors)		NPRL2 (59 tumors)		RASSF1A (36 tumors)
		AC	SCC	AC	SCC	AC	SCC
I	FD LD_av	71 (5/7) 3 (2 -- 6)	100 (11/11) 7 (2 -- 23)	14 (1/7) 2	100 (11/11) 7 (2 -- 100)	29 (2/7) 4 (3 -- 5)	33 (2/6) 4 (2 -- 8)
II	FD LD_av	60 (3/5) 5 (2 -- 9)	79 (15/19) 4 (2 -- 11)	40 (2/5) 6 (5--7)	74 (14/19) 9 (2 -- 100)	100 (1/1) 6	80 (4/5) 5 (2 -- 8)
III	FD LD_av	100 (6/6) 6 (2 -- 26)	91 (10/11) 8 (2 -- 94)	83 (5/6) 4 (2 -- 7)	91 (10/11) 10 (2 -- 100)	100 (6/6) 7 (3 -- 21)	82 (9/11) 7 (2 -- 57)
Group without metastasis	FD LD_av	70 (7/10) 3 (2 -- 6)	88 (21/24) 5 (2 -- 23)	20 (2/10) 4 (2 -- 7)	88 (21/24) 10 (2 -- 100)	29 (2/7) 4 (3 -- 6)	33 (2/6) 4 (2 -- 8)
Group with metastasis	FD LD_av	88 (7/8) 6 (2 -- 26)	88 (15/17) 7 (2 -- 94)	75 (6/8) 4 (2 -- 7)	82 (14/17) 8 (2 -- 100)	100 (7/7) 7 (3 -- 21)	81 (13/16) 6 (2 -- 57)
Total	FD LD_av	78 (14/18) 4 (2 -- 26)	88 (36/41) 6 (2 -- 94)	44 (8/18) 4 (2 -- 7)	85 (35/41) 9 (2 -- 100)	64 (9/14) 6 (3 -- 21)	68 (15/22) 6 (2 -- 57)

^a -- Frequencies of expression decrease are shown as percentages. The number of cases with decreased expression and all tested samples are shown in parentheses.
^b -- Level of expression decrease in tumor samples is shown in n-fold relative to expression in normal controls
* -- cases when decrease of target gene expression (FD and LD_av) in tumor samples relative to normal controls was statistically valid according to the non-parametric Wilcoxon test (P ≤ 0.05 -0.001)
Bold font designates cases when: (i) FD and LD_av for NPRL2 genes was more strongly pronounced in SCC than in AC samples according to the Mann -Whitney test (P = 0.002) and (ii) the difference of the FD and LD_av for RASSF1A gene was statistically valid during AC progression according to the Mann -Whitney test (P < 0.05)
Underlined italics highlights positive tendency of FD and LD_av differences of RBSP3, NPRL2 and RASSF1A genes during progression (AC and SCC respectively)

ISSN: 1471-2407