Table 2 Primers and probes for target and reference genes for expression levels and copy number studies
Gene | Name | Primers (F, R) and probe (Z) sequences 5' → 3' | Amplicon lengh |
|---|---|---|---|
Target genes | |||
RBSP3/CTDSPL NM_001008392 | RBSP3 (RB1 serine phosphatase from human chromosome 3), CTD (carboxy-terminal domain, RNA polymerase II, polypeptide A) small phosphatase-like | cDNA F: GCGAGAAAGCCTCCCAGTG R: CCACCATTCTCCTCCACCAGT Z: CCACATTGTAATCACGGAAGCAGCAGA | 154 |
| Â | Â | DNA F: CAGAGTGCGTGTGCCGACT R: ACAACTTCTCTGCGGGCGT Z: CTGGCGGAGAGACTGGGAGCGA | 126 |
NPRL2/G21 NM_006545 | Nitrogen permease regulator-like 2 gene | cDNA F: GGACCTCACTACACAACAAATCCTG R: GTCACAACGCCGTAGTACAGCA Z: ACATCCAGAAGATTTCAGCAGAGGCAGAT | 134 |
RASSF1A NM_007182 | Ras association (RalGDS/AF-6) domain family 1 | cDNA F: CGCGCATTGCAAGTTCAC R: AGGCTCGTCCACGTTCGT Z: CGCTCGTCTGCCTGGACTGTTGC | 120 |
Reference genes | Â | Â | Â |
RPN1 NM_002950 | Ribophorin I | cDNA F: CACCCTCAACAGTGGCAAGAAG R: TGCATTTCGCTCACTCTGTCG Z: CCCTCTGTCTTCAGCCTGGACTGC | 125 |
| Â | Â | DNA F: TATGGGCCTTTCAGAGATGTGCCT R: ACCACCCAAGCCTATCAACCAGTA Z: TGGAGTCCAGCCCATCCCTGTCTGCTTCA | 120 |
GUSB NM_000181 | Glucuronidase, beta β-D-glucuronidase | cDNA F: GATGGAAGAAGTGGTGCGTAGG R: TTAGAGTTGCTCACAAAGGTCACAG Z: CGTCCCACCTAGAATCTGCTGGCTACTACTT | 171 |
| Â | Â | DNA F: TGCCGTGAGTCTCTGCTGTG R: CCTACGCACCACTTCTTCCATC Z: TGACCCTCTGTCCCTTCCCTCCTG | 151 |
ACTB NM_001101 | Actin, ß | DNA F: GTGCTCAGGGCTTCTTGTCCTTT R: TTTCTCCATGTCGTCCCAGTTGGT Z: AAGGATTCCTATGTGGGCGACGAGGCCCA | 160 |
GAPDH NM_002046 | Glyceraldehyde-3-phosphate dehydrogenase | cDNA F: GGAGTCAACGGATTTGGTC R: TGGGTGGAATCATATTGGAACAT Z: CCTTCATTGACCTCAACTACATGGTTTACAT | 139 |