Figure 1

Schematic representation of the RBSP3 (A), NPRL2 / G21 / NPR2L / TUSC4 (B), and RASSF1A (C) genes. Exon-intron structure of known mRNAs is shown. Coding and non-coding mRNA regions are depicted by dark and light grey boxes, respectively. Primers and probes designed for qPCR are shown by arrows and boxes above mRNAs. A. RBSP3 (3p21.3, AP20 region). Forward primer crossed the 1^st/2^nd exons boundary, reverse primer and probe were located in the 2^nd exon. B. NPRL2 (3p21.3, LUCA region): Forward primer crossed the 5^th/6^th exons boundary, reverse primer crossed the 6^th/7^th exons boundary, probe was located in the 6^th exon. C. RASSF1A (3p21.3, LUCA region): Forward primer crossed the 1^st/2^nd exons boundary that provides specificity to isoforms A and E. Reverse primer crossed the 2^nd/3^rd exons boundary that provides specificity to isoforms A, B and D. Combination of forward and reverse primers provided specificity to isoform A only. Probe was located in the 2^nd exon.