Figure 6
From: Lipophilic aroylhydrazone chelator HNTMB and its multiple effects on ovarian cancer cells
HNTMB causes G2-Phase cell cycle arrest in SKOV-3 cells. SKOV-3 cells were treated with 0.3 or 0.6 μM HNTMB for 48 h. Cell cycle analysis of treated and untreated cells was carried out as described (Materials and Methods). Data were obtained by FACS analysis based on propidium-iodide intercalation into cellular chromatin and are presented as (A) relative fluorescence intensity in a 2-dimensional FACS profile (ModFit LT software; black lines = data line and model fit line of entire population; shaded areas = model components/subpopulations of G0/G1, S, G2/M, apoptotic cells or as (B) a table. Standardized gating was used for all samples. Ten thousand events were analyzed for each sample.