Figure 2

Variability chart (box-plots) for RT positive controls run prior to and during clinical validation stratified by gene. A standard RNA template was run across several 7900 instruments, using various primer-probe lots prior to and during clinical validation. The same RNA was included on every RT plate at the same concentration as test samples, and was used as an RT control. Nuclease-free water was used as the RT negative control. Standard deviations in aggregate cycle threshold measurements between samples ranged from 0.19 to 0.33, showing a highly controlled process.