Figure 5
Total in vitro activity of NAMPT in cell lysates. NAMPT activity as measured by conversion of C14-labelled nicotinamide to NMN by 50 μg of total protein from lysate untreated or with increasing concentrations of APO866. Absolute counts per minute (CPM) are presented. The IC50 values have been averaged from at least 3 individual experiments. Cell lines measured are A PC-3, B PC-3/TP201565, C HEK293T and D HEK293T/WT. Basal activity of PC-3/TP201565 is 4.0 fold higher than PC-3 and basal activity of HEK293T/WT is 7.4 fold higher than HEK293T. Error bars indicate SEM; IC50 values are represented with standard deviations.