Figure 6
Modulation of autophagy-related gene Beclin 1 and Bcl-2 protein expression. MDA-MB-231 [A] and MDA-MB-453 [B] cells were treated for 24 h with DMSO or C-DIMs, and whole cell lysates were isolated and analyzed by Western blot analysis according to procedures outlined in the Materials and Methods. Densitometric analyses of Beclin 1 and Bcl-2 protein expression in MDA-MB-231 and MDA-MB-453 cells was carried as described in the Materials and Methods. Results are expressed as means ± SE for three replicate determinations for each treatment group. * Significantly different from DMSO (p < 0.05). [C] Enhanced tumor staining of Beclin 1 after treatment with DIM-C-pPhC6H5 in nude mice bearing MDA-MB-231 cells as xenografts. Fixed tumor tissue from corn oil and DIM-C-pPhC6H5-treated mice [26] were stained with Beclin 1 as described in the Materials and Methods. Results are representative of tumors from two different animals per treatment group. Width of each field is 230 μm.