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Table 4 Expression of stemness markers by MGNT-derived cells forming spheres.

From: CD133, CD15/SSEA-1, CD34 or side populations do not resume tumor-initiating properties of long-term cultured cancer stem cells from human malignant glio-neuronal tumors

Samples

OCT4/POU5F1

NANOG

HTERT

CD133/PROM1

TG1

5937 a

1240

131

5

TG6

11857

1959

165

12

TG10

1686

169

517

3

TG16

871

611

73

14

  1. OCT4/POU5F1, NANOG, HTERT and CD133/PROM1 genes expressions were evaluated using real-time quantitative PCR in 4 MGNT-derived cells forming spheres. Tests were done with cultures at passage 30 ± 5. Data are from one experiment out of three giving similar results. a N-fold differences in target gene expression relative to the smallest amount of target gene mRNA detectable and quantifiable by real-time quantitative RT-PCR assays (target gene Ct value = 35; Ntarget value = 1).
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BMC Cancer

ISSN: 1471-2407

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