Figure 2
Effects of dsP21-322 on cell proliferation, colony formation and apoptosis. (A) Mock, scramble dsRNA and dsP21-322 transiently transfected cells were measured by MTT assay for the cell proliferation. (B) Mock, scramble dsRNA and dsP21-322 transfected A549 cells were treated with cisplatin and numbers of viable cells were counted by Trypan blue exclusion. (C) The mock, scramble dsRNA and dsP21-322 transfected A549 cells were seeded onto 100-mm culture dishes at a concentration of 0.5 × 103 cells and cultured for 17 days. Colony formation of dsP21-322 transfected A549 cells was inhibited obviously. (D) Apoptotic analysis by Flow Cytometry showing negative results in all of mock, scramble dsRNA and dsP21-322 transfected A549 cells.