Figure 4

Dose effect curves of gemcitabine (gem) combined with EGFR/HER2 pathway targeted therapies on TFK1 (A); EGI-1 (B); HuH28(C) and TGBC1-TKB (D). After 72 h of treatment with 1:5 scalar doses (10 μM, 2 μM, 400 nM, 80 nM, 16 nM) of gem alone or in combination with targeted therapy at a fixed 1:1 ratio, antiproliferative effect was evaluated using viability test and Chou-Talalay equation by CalcuSyn software as described in the Methods section.